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KMID : 0368419860290020085
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Journal of Plant Biology
1986 Volume.29 No. 2 p.85 ~ p.94
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On Utilization of Canavanine and Activity of Canavanase during Germination and Growth of Canavailia lineata(L.) DC
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權寧命/Kwon, Young Myung
鄭興采/高碩贊/洪英男/Chung, Hung Chae/Koh, Suck Chan/Hong, Young Nam
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Abstract
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Canavanine content of the cotyledons of Canavalia lineata decreased gradually during germination and growth of seedlings but continued to increase in roots and leaves. After abscission of cotyledons, canavanine content of leaves depleted competely. The activity of canavanase could be detected in leaves and roots, but not in cotyledons. High arginase activity was observed in the cotyledons of seeds at the early imbibition period. During the growth of seedlings, cotyledonary canavanine appeared to be transported to the growing poprtions of the seedlings where it could be utilized through nitrogen metabolic pathways. In crude cell-free extracts of leaves, maximum activities of canavanase or arginase appeared in 30mM Tris-HCl buffer (pH 9.0) or 30mM NaHCO_3 buffer (pH 10.0), respectively. The activities of these two enzymes differed from each other when treated with Co^2+ or Mn^2+. These results support the idea that canavanase and arginase might be different enzymes.
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